Peer-Reviewed Journal Details
Mandatory Fields
Cotter, PD;O'Reilly, K;Hill, C
2001
September
Journal of Food Protection
Role of the glutamate decarboxylase acid resistance system in the survival of Listeria monocytogenes LO28 in low pH foods
Validated
WOS: 73 ()
Optional Fields
ESCHERICHIA-COLI O157-H7 SALMONELLA-TYPHIMURIUM UNITED-STATES TOLERANCE GROWTH ADAPTATION IDENTIFICATION FERMENTATION ACIDULANTS RESPONSES
64
1362
1368
The glutamate decarboxylase (GAD) acid resistance system of the foodborne pathogen Listeria monocytogenes plays a major role in its survival at low pH. It was found that survival of the wild-type strain. LO28, in acidified reconstituted skim milk. diluted to reduce free glutamate levels. improves in response to supplementation with monosodium glutamate. A mutant, in which the two listerial GAD homologs have been deleted (and in which there is no discernible GAD activity), did not respond to glutamate supplementation and displayed greatly enhanced sensitivity in a number of low pH foods, even when levels of free glutamate were as low as 0.22 mM. We thus show that the GAD system plays a major role in the survival of L. monocytogenes in acidic foods even when levels of free glutamate are low.
DES MOINES
0362-028X
Grant Details