Equilibrium modified atmosphere packaging (EMAP) technology offers the possibility to maintain produce postharvest quality and extend its shelf-life. However, EMAP stability depends on well-tuned packaging design parameters to match environment conditions. This study defined design requirements of a biobased film EMAP that can preserve quality and prolong shelf-life of fresh cherry tomatoes under recommended and simulated abuse supply chain conditions. Optimum EMAP was evaluated based on headspace gas composition at 10-20 degrees C, 75-95% RH and verified by determining quality changes of packed cherry tomatoes in using a continuous or micro-perforated (0.27 mu m) bio-based intact bitter cassava (IBC) film. This was compared with a non-bio-based polymer film (oriented polypropylene, OPP). The IBC film attained equilibrium O-2 (2-3%) after 180 h at 10 degrees C, with 0 and 1 perforation, for 75 and 95% RH while OPP film maintained a downward O-2 fall. Continuous and micro-perforated IBC film did not show any major differences in equilibrium headspace O-2, thus perforation can be neglected. Based on desirability optimisation results, biobased IBC film demonstrated better optimized EMAP system in attaining recommended gas and stretching cherry tomato shelf-life as compared to non-biobased (OPP) film. The application of bio-based IBC film offers new possibilities in packaging fresh produce under equilibrium modified atmosphere without compromising their quality.