Objectives: To determine if magnesium sulphate (MgSO4) prevents lipopolysaccharide (LPS)-induced cell death in an in vitro model of the human placenta.
Study design: BeWo choriocarcinoma cells were treated with increasing concentrations of LPS (10-1000 ng/ml) and MgSO4 ranging from 1 mM to 100 mM for 24 or 48 h. For co-treatments, cells were pre-treated with I mM MgSO4 for 1 h and 200 ngiml LPS was then added for the remaining 48 h. To assess viability an mu assay was carried out along with nuclear staining to assess pyknotic nuclei. The expression of the pro-inflammatory cytokine TNF-alpha, was examined by real-time PCR.
Results: MgSO4 had a dose dependent effect of cell viability with a narrow therapeutic window. MgSO4 (1 mM) protected against adverse effects of LPS on cell viability by preventing LPS-induced decreases in cell viability (MTT assay) and protecting against LPS-induced pyknotic changes in nuclear membrane. MgSO4 also inhibited the elevated TNF-alpha mRNA expression induced by LPS.
Conclusions: MgSO4 preserves BeWo cell viability following an inflammatory insult and reduces the mRNA expression of the inflammatory cytokine TNF-alpha. These data elucidate a potential therapeutic pathway by which MgSO4 may be protective in pre-eclampsia. (C) 2016 International Society for the Study of Hypertension in Pregnancy. Published by Elsevier B.V. All rights reserved.