Sweeney, MJ;Pamies, P;Dobson, ADW

2000

May

International Journal of Food Microbiology

The use of reverse transcription-polymerase chain reaction (RT-PCR) for monitoring aflatoxin production in Aspergillus parasiticus 439

Validated

WOS: 68 ()

MOLECULAR CHARACTERIZATION GENE-TRANSCRIPTION BIOSYNTHESIS FLAVUS ASSAY AFLR EXPRESSION FAMILY LOCUS

56

97

103

A detection system based on reverse transcription PCR (RT-PCR) has been developed to monitor aflatoxin gene expression in Aspergillus pavasiticus. Total RNAs of aflatoxigenic A. parasiticus 439 grown in aflatoxin permissive and non-permissive media were amplified and monitored over time by RT-PCR with specific primers designed from two genes of the aflatoxin biosynthetic pathway. Gene transcription in both media was assessed by monitoring the house keeping beta-tubulin gene and aflatoxin production was correlated with transcription by thin layer chromatography. This RT-PCR technique has the potential to be employed as a tool to investigate the effects of a variety of physiological factors on the transcription of the aflatoxin genes. (C) 2000 Elsevier Science B.V. All rights reserved.

AMSTERDAM

0168-1605