Peer-Reviewed Journal Details
Mandatory Fields
O'Sullivan, D;Coffey, A;Fitzgerald, GF;Hill, C;Ross, RP
1998
November
Applied and Environmental Microbiology
Design of a phage-insensitive lactococcal dairy starter via sequential transfer of naturally occurring conjugative plasmids
Validated
WOS: 36 ()
Optional Fields
LACTIC-ACID BACTERIA ABORTIVE INFECTION STREPTOCOCCI RESISTANCE STRAIN BACTERIOPHAGES STRATEGY CONSTRUCTION ROTATION DEFENSES
64
4618
4622
The plasmid-free Lactococcus lactis subsp. cremoris MG1614 is highly phage sensitive and lacks lactose fermenting ability (Lac) and primary casein degrading ability (Prt). Food grade gene transfer systems were used to sequentially superimpose different phage defense systems on this background, resulting in a gradual increase in resistance to bacteriophage in the derivatives. pLP712, encoding Lac and Prt, was then transferred to one of these hosts, into which plasmids encoding adsorption inhibition, restriction modification, and abortive infection had already been introduced. This resulted in a phage-resistant strain which was successfully used as a single-strain starter for cheddar cheese manufacture under industrial conditions.
WASHINGTON
0099-2240
Grant Details