Peer-Reviewed Journal Details
Mandatory Fields
Scanlan, PD;Shanahan, F;Clune, Y;Collins, JK;O'Sullivan, GC;O'Riordan, M;Holmes, E;Wang, YL;Marchesi, JR
2008
March
Environmental Microbiology
Culture-independent analysis of the gut microbiota in colorectal cancer and polyposis
Validated
WOS: 175 ()
Optional Fields
16S RIBOSOMAL-RNA GRADIENT GEL-ELECTROPHORESIS GROUP-SPECIFIC PRIMERS FECAL MICROBIOTA HUMAN FECES INTESTINAL MICROBIOTA COLON-CANCER LACTOBACILLUS-PLANTARUM GASTROINTESTINAL-TRACT PREDOMINANT BACTERIA
10
789
798
A role for the intestinal microbiota is routinely cited as a potential aetiological factor in colorectal cancer initiation and progression. As the majority of bacteria in the gut are refractory to culture we investigated this ecosystem in subjects with colorectal cancer and with adenomatous polyposis who are at high risk of developing colorectal cancer, using culture-independent methods. Twenty colorectal cancer and 20 polypectomized volunteers were chosen for this analysis. An exploration of the diversity and temporal stability of the dominant bacteria and several bacterial subgroups was undertaken using 16S rRNA gene denaturing gradient gel electrophoresis and ribosomal intergenic spacer analysis (RISA). Metabonomic analysis of the distal gut microbiota's environment was also undertaken. A significantly reduced temporal stability and increased diversity for the microbiota of subjects with colorectal cancer and polyposis was evident. A significantly increased diversity of the Clostridium leptum and C. coccoides subgroups was also noted for both disease groups. A clear division in the metabonome was observed for the colorectal cancer and polypectomized subjects compared with control volunteers. The intestinal microbiota and their metabolites are significantly altered in both colorectal cancer and polypectomized subjects compared with controls.
OXFORD
1462-2912
10.1111/j.1462-2920.2007.01503.x
Grant Details