Peer-Reviewed Journal Details
Mandatory Fields
Loughran, G;Howard, MT;Firth, AE;Atkins, JF
2017
August
Rna-A Publication of The Rna Society
Avoidance of reporter assay distortions from fused dual reporters
Validated
WOS: 27 ()
Optional Fields
VEGF-AX CLEAVAGE STOP 2A CODON TRANSLATION POLYPROTEIN APHTHOVIRUS READTHROUGH FUSION
23
1285
1289
Positioning test sequences between fused reporters permits monitoring of both translation levels and framing, before and after the test sequence. Many studies, including those on recoding such as productive ribosomal frameshifting and stop codon readthrough, use distinguishable luciferases or fluorescent proteins as reporters. Occasional distortions, due to test sequence product interference with the individual reporter activities or stabilities, are here shown to be avoidable by the introduction of tandem StopGo sequences (2A) flanking the test sequence. Using this new vector system (pSGDluc), we provide evidence for the use of a 3' stem-loop stimulator for ACP2 readthrough, but failed to detect the reported VEGFA readthrough.
COLD SPRING HARBOR
1355-8382
10.1261/rna.061051.117
Grant Details