Peer-Reviewed Journal Details
Mandatory Fields
Firth, AE,Atkins, JF;
2009
September
Virology Journal
Evidence for a novel coding sequence overlapping the 5 '-terminal similar to 90 codons of the Gill-associated and Yellow head okavirus envelope glycoprotein gene
Validated
()
Optional Fields
PENAEUS-MONODON PRAWNS GENOME SEQUENCE NIDOVIRUS RNA SHRIMP VIRUS TRANSCRIPTION ALIGNMENT COMPLEX GP116
6
The genus Okavirus (order Nidovirales) includes a number of viruses that infect crustaceans, causing major losses in the shrimp industry. These viruses have a linear positive-sense ssRNA genome of similar to 26-27 kb, encoding a large replicase polyprotein that is expressed from the genomic RNA, and several additional proteins that are expressed from a nested set of 3'-coterminal subgenomic RNAs. In this brief report, we describe the bioinformatic discovery of a new, apparently coding, ORF that overlaps the 5' end of the envelope glycoprotein encoding sequence, ORF3, in the +2 reading frame. The new ORF has a strong coding signature and, in fact, is more conserved at the amino acid level than the overlapping region of ORF3. We propose that translation of the new ORF initiates at a conserved AUG codon separated by just 2 nt from the ORF3 AUG initiation codon, resulting in a novel 86 amino acid protein.
ARTN 222
Grant Details