Tedesco, S,Doyle, H,Blasco, J,Redmond, G,Sheehan, D;

2010

January

Comparative Biochemistry And Physiology C-Pharmacology Toxicology & Endocrinology

Exposure of the blue mussel, Mytilus edulis, to gold nanoparticles and the pro-oxidant menadione

Validated

()

Mytilus edulis Nanoparticles Menadione Oxidative stress Thiol proteins Toxicity Glutathione Proteomic Gold OXIDATIVE STRESS THIOREDOXIN REDUCTASE PROTEOMIC IDENTIFICATION TITANIUM-DIOXIDE FEEDING RESPONSE PROTEIN THIOLS DAPHNIA-MAGNA GLUTATHIONE TOXICITY TARGET

151

167

174

Relatively little is known about how gold nanoparticles (GNP) might interact in vivo with marine organisms. Mytilus edulis was exposed (24 h) to similar to 15 nm GNP, menadione and both compounds simultaneously (GNP/menadione). GNP was detected by inductively coupled plasma-optical emission spectroscopy mainly in digestive gland of samples exposed to GNP though not GNP/menadione, perhaps due to impaired feeding. Thioredoxin reductase activity and malondialdehyde levels were determined in all tissues. Thioredoxin reductase inhibition was detected only in digestive gland exposed to menadione whilst malondialdehyde levels did not vary in response to treatment in all tissues. GNP caused a decrease in the reduced/oxidized glutathione ratio in digestive gland, but no difference was found in other tissues or for other treatments. One dimensional electrophoresis of proteins containing thiol groups was performed in all tissues and revealed a reduction in protein thiols for all treatments in digestive gland. Two dimensional electrophoresis of digestive gland extracts, from GNP and control groups, showed decreased levels of thiol proteins in response to GNP which we attribute to oxidation. Our results suggest that GNP causes a modest level of oxidative stress sufficient to oxidize thiols in glutathione and proteins but without causing lipid peroxidation or induction of thioredoxin reductase activity. (C) 2009 Elsevier Inc. All rights reserved.

DOI 10.1016/j.cbpc.2009.10.002