Peer-Reviewed Journal Details
Mandatory Fields
Melian, EB,Hinzman, E,Nagasaki, T,Firth, AE,Wills, NM,Nouwens, AS,Blitvich, BJ,Leung, J,Funk, A,Atkins, JF,Hall, R,Khromykh, AA;
2010
January
Journal of Virology
NS1 ' of Flaviviruses in the Japanese Encephalitis Virus Serogroup Is a Product of Ribosomal Frameshifting and Plays a Role in Viral Neuroinvasiveness
Validated
()
Optional Fields
WEST-NILE RNA REPLICATION KUNJIN VIRUS PROTEIN SEQUENCE SIGNAL TRANSCOMPLEMENTATION PSEUDOKNOT ANTIBODIES SUGGESTS
84
1641
1647
Flavivirus NS1 is a nonstructural protein involved in virus replication and regulation of the innate immune response. Interestingly, a larger NS1-related protein, NS1', is often detected during infection with the members of the Japanese encephalitis virus serogroup of flaviviruses. However, how NS1' is made and what role it performs in the viral life cycle have not been determined. Here we provide experimental evidence that NS1' is the product of a -1 ribosomal frameshift event that occurs at a conserved slippery heptanucleotide motif located near the beginning of the NS2A gene and is stimulated by a downstream RNA pseudoknot structure. Using site-directed mutagenesis of these sequence elements in an infectious clone of the Kunjin subtype of West Nile virus, we demonstrate that NS1' plays a role in viral neuroinvasiveness.
DOI 10.1128/JVI.01979-09
Grant Details