Peer-Reviewed Journal Details
Mandatory Fields
Casanova, E,Guetg, N,Vigot, R,Seddik, R,Julio-Pieper, M,Hyland, NP,Cryan, JF,Gassmann, M,Bettler, B;
2009
February
Genesis
A Mouse Model for Visualization of GABA(B) Receptors
Validated
()
Optional Fields
enhanced GFP enteric GABA-B GABA(B) GABA(B1) GPCR transgenic HIPPOCAMPAL-NEURONS ESCHERICHIA-COLI TRANSGENIC MICE RAT PROTEIN RECOMBINATION BRAIN LOCALIZATION EXPRESSION DIVERSITY
47
595
602
GABA(B) receptors are the G-protein-coupled receptors for the neurotransmitter gamma-aminobutyric acid (GABA). Receptor subtypes are based on the subunit isoforms GABA(B1a) and GABA(B1b), which combine with GABA(B2) subunits to form heteromeric receptors. Here, we used a modified bacterial artificial chromosome (BAC) containing the GABA(B1) gene to generate transgenic mice expressing GABA(B1). and GABA(B1b) subunits fused to the enhanced green fluorescence protein (eGFP). We demonstrate that the GABA(B1)-eGFP fusion proteins reproduce the cellular expression patterns of endogenous GABA(B1) proteins in the brain and in peripheral tissue. Crossing the GABA(B1)-eGFP BAC transgene into the GABA(B1)(-/-) background restores pre and postsynaptic GABA(B) functions, showing that the GABA(B1)-eGFP fusion proteins substitute for the lack of endogenous GABA(B1) proteins. Finally, we demonstrate that the GABA(B1)-eGFP fusion proteins replicate the temporal expression patterns of native GABA(B) receptors in cultured neurons. These transgenic mice therefore provide a validated tool for direct visualization of native GABA(B) receptors. genesis 47:595-602,2009. (C) 2009 Wiley-Liss, Inc.
DOI 10.1002/dvg.20535
Grant Details