Peer-Reviewed Journal Details
Mandatory Fields
Kompanowska-Jezierska, E,Wolff, H,Kuczeriszka, M,Gramsbergen, JB,Walkowska, A,Johns, EJ,Bie, P;
2008
April
American Journal of Physiology
Renal nerves and nNOS: roles in natriuresis of acute isovolumetric sodium loading in conscious rats
Validated
()
Optional Fields
plasma renin concentration total body sodium blood pressure sodium excretion NITRIC-OXIDE SYNTHASE MACULA DENSA NOS RENIN SECRETION BLOOD-FLOW TUBULOGLOMERULAR FEEDBACK ARTERIAL-PRESSURE WATER-EXCRETION NERVOUS-SYSTEM DOGS RELEASE
294
1130
1139
It was hypothesized that renal sympathetic nerve activity (RSNA) and neuronal nitric oxide synthase (nNOS) are involved in the acute inhibition of renin secretion and the natriuresis following slow NaCl loading (NaLoad) and that RSNA participates in the regulation of arterial blood pressure (MABP). This was tested by NaLoad after chronic renal denervation with and without inhibition of nNOS by S-methyl-thiocitrulline (SMTC). In addition, the acute effects of renal denervation on MABP and sodium balance were assessed. Rats were investigated in the conscious, catheterized state, in metabolic cages, and acutely during anesthesia. NaLoad was performed over 2 h by intravenous infusion of hypertonic solution (50 mu mol(.)min(-1.)kg body mass(-1)) at constant body volume conditions. SMTC was coinfused in amounts (20 mu g(.)min(-1.)kg(-1)) reported to selectively inhibit nNOS. Directly measured MABPs of acutely and chronically denervated rats were less than control (15% and 9%, respectively, P < 0.005). Plasma renin concentration (PRC) was reduced by renal denervation (14.5 +/- 0.2 vs. 19.3 +/- 1.3 mIU/l, P < 0.005) and by nNOS inhibition (12.4 +/- 2.3 vs. 19.6 +/- 1.6 mlU/l, P < 0.005). NaLoad reduced PRC (P < 0.05) and elevated MABP modestly (P < 0.05) and increased sodium excretion six-fold, irrespective of renal denervation and SMTC. The metabolic data demonstrated that renal denervation lowered sodium balance during the first days after denervation (P < 0.001). These data show that renal denervation decreases MABP and renin secretion. However, neither renal denervation nor nNOS inhibition affects either the renin down-regulation or the natriuretic response to acute sodium loading. Acute sodium-driven renin regulation seems independent of RSNA and nNOS under the present conditions.
DOI 10.1152/ajpregu.00908.2007
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