Peer-Reviewed Journal Details
Mandatory Fields
Cronin, M,Knobel, M,O'Connell-Motherway, M,Fitzgerald, GF,van Sinderen, D;
2007
July
Applied and Environmental Microbiology
Molecular dissection of a Bifidobacterial replicon
Validated
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Optional Fields
COMPLETE NUCLEOTIDE-SEQUENCE INITIATOR PROTEIN REP LACTIC-ACID BACTERIA CANCER GENE-THERAPY DELIVERY-SYSTEM CRYPTIC PLASMID VECTOR CONSTRUCTION ESCHERICHIA-COLI DNA-REPLICATION BREVE UCC2003
73
7858
7866
The 2.1-kb cryptic plasmid pCIBAO89 from Bifidobacterium asteroides harbors a 1.4-kb segment which is sufficient for its autonomous replication. The segment is divided into two parts, the presumed replication origin, ori89, and the rep gene encoding the putative 41-kDa Rep89 replication initiation protein. This minimal replication region of pCIBAO89 was functionally dissected by transcriptional analyses as well as by DNA-binding studies, and the information obtained was exploited to create a number of Escherichia coli-Bifidobacterium shuttle vectors capable of transforming various bifidobacteria with an efficiency of up to 106 transformants/mu g DNA.
DOI 10.1128/AEM.01630-07
Grant Details