Peer-Reviewed Journal Details
Mandatory Fields
Atkins, JF,Wills, NM,Loughran, G,Wu, CY,Parsawar, K,Ryan, MD,Wang, CH,Nelson, CC;
2007
March
Rna-A Publication of The Rna Society
A case for "StopGo'': Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go)
Validated
()
Optional Fields
codon redefinition recoding ribosome picorna 2A StopGo PICORNA-LIKE-VIRUS PEPTIDYL TRANSFERASE CENTER TRANSFER-RNA AMINO-ACID NASCENT PEPTIDE MESSENGER-RNA A-SITE CLEAVAGE ACTIVITIES ECTROPIS-OBLIQUA BOND FORMATION
13
803
810
When a eukaryotic mRNA sequence specifying an amino acid motif known as 2A is directly followed by a proline codon, two nonoverlapping proteins are synthesized. From earlier work, the second protein is known to start with this proline codon and is not created by proteolysis. Here we identify the C-terminal amino acid of an upstream 2A-encoded product from Perina nuda picorna-like virus that is glycine specified by the last codon of the 2A-encoding sequence. This is an example of recoding where 2A promotes unconventional termination after decoding of the glycine codon and continued translation beginning with the 39 adjacent proline codon.
DOI 10.1261/rna.487907
Grant Details