Peer-Reviewed Journal Details
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Mullen, A,Moloney, F,Nugent, AP,Doyle, L,Cashman, KD,Roche, HM;
2007
July
Journal of Nutritional Biochemistry
Conjugated linoleic acid supplementation reduces peripheral blood mononuclear cell interleukin-2 production in healthy middle-aged males
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conjugated linoleic acid supplementation peripheral blood mononuclear cell interleukin-2 C-REACTIVE PROTEIN KAPPA-B ACTIVATION MODERATELY OVERWEIGHT SUBJECTS CORONARY-HEART-DISEASE TUMOR-NECROSIS-FACTOR LDL-PHENOTYPE-B IMMUNE FUNCTION DIETARY SUPPLEMENTATION CYTOKINE PRODUCTION COX-2 EXPRESSION
18
658
666
Conjugated linoleic acid (CLA) refers to geometric and positional isomers of linoleic acid. Animal studies have shown that CLA modulates the immune system and suggest that it may have a therapeutic role in inflammatory disorders. This double-blind placebo-controlled intervention trial investigated the effects of CLA supplementation on indices of immunity relating to cardiovascular disease (CVD) in a cohort of healthy middle-aged male volunteers. Subjects were randomly assigned to supplement their diet with 2.2 g 50:50 isomeric blend of cis 9. trans 11 (c9, t11)-CLA and trans 10, cis 12 (t10, c12)-CLA or placebo daily for 8 weeks.Interleukin (IL) 2, IL-10 and tumour necrosis factor (TNF) alpha were measured in the supernatant of cultured unstimulated and concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC) by ELISA. Serum IL-6 and plasma CRP were measured by ELISA and plasma fibrinogen by automated clotting assay. Gene expression was investigated by real-time RT-PCR.CLA supplementation significantly reduced Con A-stimulated PBMC IL-2 secretion (37.1%; P=.02). CLA supplementation had no significant effect on transcription of IL-2. CLA supplementation had no direct significant effects on PBMC TNF alpha or IL-10 secretion. Other inflammatory markers associated with CVD, including IL-6, CRY and fibrinogen, were not affected by CLA supplementation.This study showed that CLA supplementation reduced PBMC IL-2 secretion from Con A-stimulated PBMC but lacked effect on other markers of the human inflammatory response. (c) 2007 Elsevier Inc. All rights reserved.
DOI 10.1016/j.jnutbio.2006.12.008
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