Peer-Reviewed Journal Details
Mandatory Fields
Lillis, B,Manning, M,Hurley, E,Berney, H,Duane, R,Mathewson, A,Sheehan, MM;
2007
February
Biosensors & Bioelectronics
Investigation into the effect that probe immobilisation method type has on the analytical signal of an EIS DNA biosensor
Validated
()
Optional Fields
electrolyte insulator semiconductor DNA biosensor impedance spectroscopy DNA sensor DNA immobilisation HYBRIDIZATION SILICON SURFACE SEQUENCES CHIPS
22
1289
1295
The analytical performance of an enhanced surface area electrolyte insulator semiconductor (EIS) device was investigated for DNA sensor development. The work endeavored to advance EIS performance by monitoring the effect of DNA probe layers have on the impedimetric signal during target hybridisation detection. Two universally employed covalent chemistries, direct and spacer-mediated attachment of amino modified probe molecules to antino-functionalised surfaces were investigated. Relative areal densities of immobilised probe were measured on planar and enhanced surface area substrates using epi-fluorescence microscopy. The reproducibility of the each immobilisation method was seen to have a direct effect on the reproducibility of the impedimetric signal. The sensitivity and selectivity was seen to be dependent on the type of immobilisation method. Real time, impedimetric detection of target DNA hybridisation concentrations as low as 25 and 1 nM were possible. The impact that probe concentration had on the impedimetric signal for selective and non-selective interactions was also investigated. (c) 2006 Elsevier B.V. All rights reserved.
DOI 10.1016/j.bios.2006.05.021
Grant Details