Peer-Reviewed Journal Details
Mandatory Fields
Clarke, G,Mahony, S,Malone, G,Dinan, TG;
2007
March
Journal of Neuroscience Methods
An isocratic high performance liquid chromatography method for the determination of GABA and glutamate in discrete regions of the rodent brain
Validated
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Optional Fields
GABA glutamate high performance liquid chromatography naphthalene-2,3-dicarboxaldehyde isocratic protocol maternal deprivation AMINO-ACID NEUROTRANSMITTERS MATERNAL SEPARATION ELECTROCHEMICAL DETECTION RAT HIPPOCAMPUS ANXIETY STRESS DISORDERS BEHAVIORS RECEPTORS RESPONSES
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The amino acid neurotransmitters gamma-aminobutyric acid (GABA) and glutamate have been implicated in mood disorders and high performance liquid chromatography (HPLC) is an important method in their quantitation. This paper describes a method which employs either electrochemical or fluorescent detection of the amino acid derivatives formed by a reaction with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide ions. Elution is based on an isocratic protocol and is achieved in 20 min or less. The method is reproducible, shows excellent linearity and generates derivatives which are stable for up to 16 h. Successful application of the method to a maternal deprivation study has identified the hippocampus and the brainstem as possible sites of long-term alteration in this paradigm. (c) 2006 Elsevier B.V. All rights reserved.
DOI 10.1016/j.jneumeth.2006.09.006
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