Peer-Reviewed Journal Details
Mandatory Fields
Mc Grath, S,Neve, H,Seegers, JFML,Eijlander, R,Vegge, CS,Brondsted, L,Heller, KJ,Fitzgerald, GF,Vogensen, FK,van Sinderen, D;
2006
June
Journal of Bacteriology
Anatomy of a lactococcal phage tail
Validated
()
Optional Fields
BACTERIOPHAGE-LAMBDA TAIL LACTIC-ACID BACTERIA STRUCTURAL PROTEINS TEMPERATE BACTERIOPHAGE-TP901-1 MOLECULAR CHARACTERIZATION TRANSLATIONAL FRAMESHIFT COMPARATIVE GENOMICS DNA-SEQUENCE IDENTIFICATION GENES
188
3972
3982
Bacteriophages of the Siphoviridae family utilize a long noncontractile tail to recognize, adsorb to, and inject DNA into their bacterial host. The tail anatomy of the archetypal Siphoviridae X has been well studied, in contrast to phages infecting gram-positive bacteria. This report outlines a detailed anatomical description of a typical member of the Siphoviridae infecting a gram-positive bacterium. The tail superstructure of the lactococcal phage Tuc2009 was investigated using N-terminal protein sequencing, Western blotting, and immunogold transmission electron microscopy, allowing a tangible path to be followed from gene sequence through encoded protein to specific architectural structures on the Tuc2009 virion. This phage displays a striking parity with X with respect to tail structure, which reenforced a model proposed for Tuc2009 tail architecture. Furthermore, comparisons with X and other lactococcal phages allowed the specification of a number of genetic submodules likely to encode specific tail structures.
DOI 10.1128/JB.00024-06
Grant Details