Peer-Reviewed Journal Details
Mandatory Fields
Hesari, J,Ehsani, MR,McSweeney, PLH;
2006
June
Milchwissenschaft-Milk Science International
The influence of whey proteins on peptidase activities of Lactococcus lactis spp. cremoris AM1
Validated
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Optional Fields
ENZYME-ACTIVITY AMINOPEPTIDASE PLASMIN CHEESE MILK PURIFICATION PROTEOLYSIS SUBSTRATE CHYMOSIN MODEL
61
316
318
The effect of the native whey proteins alpha-lactalbumin (alpha-La), P-lactoglobulin (beta-Lg) and bovine serum albumin (BSA) on the aminopepticlase activity of Lactococcus (Lc.) lactis spp. cremoris AM1 was investigated using p-nitroanilide (NA) derivatives of amino acids as substrates. PepN-like activity was decreased by inclusion of mixed whey proteins. While a-La did not have any effect on PepN-like activity, this activity was inhibited by beta-Lg and BSA. In contrast, increased hydrolysis of Ala-Pro-pNA and Pro-pNA (specific substrates for PepX and Pepl, respectively) were observed on inclusion of whey proteins. Peptidase activity on Glu-pNA as substrate was not affected by the whey protein mixture, although it was decreased by alpha-La and increased by BSA. beta-Lg did not have significant effect on PepA-like activity as assayed on Glu-pNA.
Grant Details