Peer-Reviewed Journal Details
Mandatory Fields
Sheehan, VM,Sleator, RD,Fitzgerald, GF,Hill, C;
2006
March
Applied and Environmental Microbiology
Heterologous expression of BetL, a betaine uptake system, enhances the stress tolerance of Lactobacillus salivarius UCC118
Validated
()
Optional Fields
LACTIC-ACID BACTERIA LISTERIA-MONOCYTOGENES LO28 GLYCINE BETAINE LACTOCOCCUS-LACTIS TRANSPORT-SYSTEM PROBIOTIC BACTERIA COMPATIBLE SOLUTES GENE-EXPRESSION SALT TOLERANCE NISA PROMOTER
72
2170
2177
Given the increasing commercial and clinical relevance of probiotic cultures, improving the technological robustness of what are often process-sensitive cultures is an important biological goal. The nisin-controlled expression system was used to direct the heterologous expression of the listerial betaine uptake system BetL in the probiotic strain Lactobacillus salivarius UCC118. Following nisin induction, strains expressing betL exhibited a significant increase in resistance to several stresses, including elevated osmo-, cryo-, baro-, and chill tolerance, as well as increased resistance to spray- and freeze-drying. The ability to confer additional stress tolerance on a probiotic culture may be an important step in delivering viable cultures for maximal efficacy.
DOI 10.1128/AEM.72.3.2170-2177.2006
Grant Details