Peer-Reviewed Journal Details
Mandatory Fields
Zotta, T,Piraino, P,Ricciardi, A,McSweeney, PLH,Parente, E;
2006
April
Food Chemistry
Proteolysis in model sourdough fermentations
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Optional Fields
wheat sourdoughs proteolysis lactic acid bacteria yeasts LACTIC-ACID BACTERIA ASPARTIC PROTEINASE GLUTEN HYDROLYSIS WHEAT GLUTEN AMINO-ACIDS DOUGH DEPOLYMERIZATION FINGERPRINTS LACTOBACILLI PURIFICATION
54
2567
2574
Model wheat doughs started with six different lactic acid bacteria (LAB), with or without a commercial baker's yeast culture, were used to study proteolysis in sourdough fermentations. Cell counts, pH, and free amino acid concentration were measured. Sequential extraction of dough samples was performed to separate wheat proteins. The salt-soluble protein fraction (albumins and globulins) was analyzed by RP-HPLC and SDS-PAGE, whereas propanol-soluble (gliadins) and insoluble (glutenins) protein fractions were analyzed by SDS-PAGE only. Multivariate statistical methods were used for the analysis of results. The presence of yeasts and LAB affected RP-HPLC and SDS-PAGE patterns of the salt-soluble fraction in a complex way. The only changes in the gluten proteins that could be related to the presence of LAB were the appearance of new protein fragments (20 and 27 kDa) from gliadins and the degradation of high molecular weight glutenin subunits.
DOI 10.1021/jf052504s
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