Peer-Reviewed Journal Details
Mandatory Fields
Ventura, M,Fitzgerald, GF,van Sinderen, D;
2005
May
Applied and Environmental Microbiology
Genetic and transcriptional organization of the clpC locus in Bifidobacterium breve UCC 2003
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Optional Fields
GRAM-POSITIVE BACTERIA HEAT-SHOCK RESPONSE MOLECULAR CHAPERONES LACTOCOCCUS-LACTIS STRESS-RESPONSE UCC 2003 EXPRESSION PROTEINS REGULATOR OPERON
71
6282
6291
A homolog of the clpC ATPase gene was identified in the genome of Bifidobacterium breve UCC 2003. Since this gene is very well conserved among eubacteria, we employed a PCR-based approach using primers based on highly conserved regions of ClpC proteins in order to identify homologous genes in other bifidobacterial species. Analysis by slot blot, Northern blot, and primer extension experiments showed that transcription of c1pC is induced in response to moderate heat shock regimes. Moreover, we identified in the genome sequence of B. breve UCC 2003 a gene, designated clgR, which is predicted to encode a transcriptional regulator involved in regulation of the bifidobacterial clpC gene. The role of this protein in the regulation of B. breve UCC 2003 clpC gene expression was investigated by performing gel retardation experiments. We show that a biologically active ClgR molecule requires one or more proteinaceous coactivators to assist in the specific binding of ClgR to the clpC promoter region.
DOI 10.1128/AEM.71.10.6282-6291.2005
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