Peer-Reviewed Journal Details
Mandatory Fields
Bagnall, NM,Dent, PC,Walkowska, A,Sadowski, J,Johns, EJ;
2005
May
Journal of Physiology-London
Nitric oxide inhibition and the impact on renal nerve-mediated antinatriuresis and antidiuresis in the anaesthetized rat
Validated
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Optional Fields
NOREPINEPHRINE OVERFLOW FLUID REABSORPTION HYPERTENSIVE-RATS VOLUME EXPANSION L-ARGININE SYNTHASE STIMULATION KIDNEY VASCULATURE MODULATION
569
849
856
The contribution of nitric oxide (NO) to the antinatriuresis and antidiuresis caused by low-level electrical stimulation of the renal sympathetic nerves (RNS) was investigated in rats anaesthetized with chloralose-urethane. Groups of rats, n= 6, were given IV infusions of vehicle, L-NAME (10 mu g kg(-1) min(-1)), 1400W (20 mu g kg(-1) min(-1)), or S-methyl-thiocitrulline (SMTC) (20 mu g kg(-1) min(-1)) to inhibit NO synthesis non-selectively or selectively to block the inducible or neuronal NOS isoforms (iNOS and nNOS, respectively). Following baseline measurements of blood pressure (BP), renal blood flow (RBF), glomerular filtration rate (GFR), urine flow (UV) and sodium excretion (UNaV), RNS was performed at 15 V, 2 ms duration with a frequency between 0.5 and 1.0 Hz. RNS did not cause measurable changes in BP, RBF or GFR in any of the groups. In untreated rats, RNS decreased UV and UNaV by 40-50% (both P < 0.01), but these excretory responses were prevented in L-NAME-treated rats. In the presence of 1400W IV , RNS caused reversible reductions in both UV and UNaV of 40-50% (both P < 0.01), while in SMTC-treated rats, RNS caused an inconsistent fall in UV, but a significant reduction (P < 0.05) in UNaV of 21%. These data demonstrated that the renal nerve-mediated antinatriuresis and antidiuresis was dependent on the presence of NO, generated in part by nNOS. The findings suggest that NO importantly modulates the neural control of fluid reabsorption; the control may be facilitatory at a presynaptic level but inhibitory on tubular reabsorptive processes.
DOI 10.1113/jphysiol.2005.097709
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