Peer-Reviewed Journal Details
Mandatory Fields
Cusack, S,Jewell, C,Cashman, KD;
2005
January
Journal of Prostaglandins
The effect of conjugated linoleic acid on the viability and metabolism of human osteoblast-like cells
Validated
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Optional Fields
GROWTH-FACTOR-I FATTY-ACIDS PROSTAGLANDIN E-2 BODY-COMPOSITION CACO-2 CELLS CANCER CELLS APOPTOSIS RATS ISOMERS CIS-9
72
29
39
Studies in experimental animals and murine osteoblast cells in culture have produced conflicting findings on the effect of conjugated linoleic acid (CLA) on bone formation. The present study investigated the influence of CLA on viability and metabolism of two human osteoblast-like cell lines (SaOS2 and MG63). Both cell lines were exposed to increasing concentrations (0-50 muM) of CLA either as pure cis (c) 9: trans (t) 11 and t10:c12 CLA isomers or a blend of isomers, or linoleic acid (C18:2). Cell cytotoxicity and degree of DNA fragmentation were unaffected by any fatty acid treatment. PGE(2) biosynthesis by both cell lines was variably reduced by CLA isomer blend and t10:c12 CLA, but not c9:t11 CLA. Alkaline phosphatase activity was variably increased by all CLA treatments. These results suggest a lack of cytotoxic effect of CLA on human osteoblast-like cells and tentatively suggest a possible beneficial effect on bone formation in humans. (C) 2004 Elsevier Ltd. All rights reserved.
DOI 10.1016/j.plefa.2004.09.002
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