Peer-Reviewed Journal Details
Mandatory Fields
Stapleton, PC,O'Mahony, J,Dobson, ADW;
2004
February
Canadian Journal of Microbiology
Real-time PCR analysis of carbon catabolite repression of cellobiose dehydrogenase gene transcription in Trametes versicolor
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cellobiose dehydrogenase carbon repression real-time PCR ASPERGILLUS-NIDULANS PHANEROCHAETE-CHRYSOSPORIUM TRICHODERMA-REESEI BINDING-SITES CLONING DEGRADATION EXPRESSION INDUCTION CELLULASE CLUSTER
50
113
119
Cellobiose dehydrogenase production in Trametes versicolor is repressed when additional carbon sources, such as glucose, maltose, galactose, arabinose, and xylose, are added to the fungal cultures growing on cellulose. Realtime quantitative reverse transcription - polymerase chain reaction has been used to demonstrate that the addition of galactose, arabinose, and xylose results in 19-, 92-, and 114-fold reductions, respectively, in cdh transcript levels 96 h post-addition. Glucose exhibits the greatest repressive effect, resulting in a 3400-fold decrease in cdh transcript levels.
DOI 10.1139/W03-108
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