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Lan, W,Harmon, D,Wang, JH,Shorten, G,Redmond, P;
European Journal of Anaesthesiology
The effect of lidocaine on neutrophil CD11b/CD18 and endothelial ICAM-1 expression and IL-1 beta concentrations induced by hypoxia-reoxygenation
Optional Fields
anaesthetics local lidocaine endothelial cell interleukins cytokines inflammation hypoxia reoxygenation neutrophil cytokines ISCHEMIA-REPERFUSION LIPID-PEROXIDATION LOCAL-ANESTHETICS INJURY CELLS
Background: Lidocaine has actions potentially of benefit during ischaemia-reperfusion. Neutrophils and endothelial cells have an important role in ischaemia-reperfusion injury.Methods: Isolated human neutrophil CD11b and CD18, and human umbilical vein endothelial cell (HUVEC) ICAM-1 expression and supernatant IL-1beta concentrations in response to hypoxia-reoxygenation were studied in the presence or absence of different concentrations of lidocaine (0.005, 0.05 and 0.5 mg mL(-1)). Adhesion molecule expression was quantified by flow cytometry and IL-1beta concentrations by ELISA. Differences were assessed with analysis of variance and Student-Newman-Keuls as appropriate. Data are presented as mean +/- SD.Results: Exposure to hypoxia-reoxygenation increased neutrophil CD11b (94-33 +/- 40.65 vs. 34.32 +/- 6.83 mean channel fluorescence (MCF), P = 0,02), CD18 (109.84 +/- 35.44 vs. 59.05 +/- 6.71 MCF, P = 0.03) and endothelial ICAM-1 (146.62 +/- 16.78 vs. 47.29 +/- 9.85 MCF, P < 0.001) expression compared to normoxia. Neutrophil CD18 expression on exposure to hypoxia-reoxygenation was less in lidocaine (0.005 mgmL-1) treated cells compared to control (71.07 +/- 10.14 vs. 109.84 +/- 35.44 MCF, P = 0.03). Endothelial ICAM-1 expression on exposure to hypoxia-reoxygenation was less in lidocalne (0.005 mg mL(-1)) treated cells compared to control (133.25 +/- 16.05 vs. 146.62 - 16.78 MCF, P = 0.03). Hypoxia-reoxygenation increased HUVEC supernatant IL-1beta concentrations compared to normoxia (3.41 +/- 0.36 vs. 2.65 +/- 0.21 pg mL(-1), P = 0.02). Endothelial supernatant IL-1beta concentrations in lidocaine-treated HUVECs were similar to controls.Conclusions: Lidocaine at clinically relevant concentrations decreased neutrophil CD18 and endothelial ICAM-I expression but not endothelial IL-Ibeta concentrations.
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