Peer-Reviewed Journal Details
Mandatory Fields
Forde, A,Fitzgerald, GF;
2003
March
Plasmid
Molecular organization of exopolysaccharide (EPS) encoding genes on the lactococcal bacteriophage adsorption blocking plasmid, pCI658
Validated
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Optional Fields
exopolysaccharide plasmid genetic analysis bacteriophage resistance LACTIC-ACID BACTERIA/ VIBRIO-CHOLERAE O1 STRUCTURAL-CHARACTERIZATION CAPSULAR POLYSACCHARIDE SEQUENCE-ANALYSIS MICROBIAL EXOPOLYSACCHARIDES GLUCOSE-DEHYDROGENASE FUNCTIONAL-ANALYSIS SHIGELLA-FLEXNERI ESCHERICHIA-COLI
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The lactococcal plasmid pCI1658 (58 kb) isolated from Lactococcus lactis ssp. cremoris HO2 encodes the production of a hydrophilic exopolysaccharide (EPS) which consists primarily of galactose and glucuronic acid and which interferes with adsorption of phages phi712 and phic2 to cell surface receptors. Examination of the nucleotide sequence of a 21.8-kb region of the plasmid revealed a large genetic cluster consisting of at least 23 putative EPS biosynthetic determinants in addition to the presence of insertion sequences at the 5' and 3' ends. According to homology searches, the genes were organized in specific regions involved in regulation, synthesis and export of the EPS. The predicted products of individual genes exhibited significant homology to exopolysaccharide, capsular polysaccharide (CPS), and lipopolysaccharide (LPS) gene products from a variety of Gram positive and Gram negative bacteria. Evidence of a gene encoding UDP-glucose dehydrogenase is also presented and this is the first description of such a gene in Lactococcus. (C) 2002 Published by Elsevier Science (USA).
DOI 10.1016/S0147-619X(02)00156-7
Grant Details