Peer-Reviewed Journal Details
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Tangney, M,Fitzgerald, GF;
2002
February
Fems Microbiology Letters
Effectiveness of the lactococcal abortive infection systems AbiA, AbiE, AbiF and AbiG against P335 type phages
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P335 AbiA AbiE AbiF AbiG abortive infection mechanism LACTIC-ACID BACTERIA DEFENSE SYSTEMS DNA FRAGMENTS RAPID METHOD BACTERIOPHAGES RESISTANCE MECHANISMS DIFFERENTIATION HYBRIDIZATION STREPTOCOCCI
210
67
72
Four lactococcal abortive infection mechanisms were introduced into strains which were sensitive hosts for P335 type phages and plaque assay experiments performed to assess their effect on five lactococcal bacteriophages from this family. Results indicate that AbiA inhibits all five P335 phages tested, while AbiG affects phiP335 itself and phiQ30 but not the other P335 species phages. AbiA was shown to retard phage Q30 DNA replication as previously reported for other phages. It was also demonstrated that AbiG, previously shown to act at a point after DNA replication in the cases of c2 type and 936 type phages, acts at the level of, or prior to phage Q30 DNA replication. AbiE and AbiF had no effect on the P335 type phages examined. C 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
PII S0378-1097(02)00575-X
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