Peer-Reviewed Journal Details
Mandatory Fields
Labarre, C,Schirawski, J,van der Zwet, A,Fitzgerald, GF,van Sinderen, D;
Fems Microbiology Letters
Insertional mutagenesis of an industrial strain of Streptococcus thermophilus
Optional Fields
mutagenesis industrial microorganism oligopeptide transport Streptococcus thermophilus OLIGOPEPTIDE TRANSPORT PLASMID SYSTEM GENES
Random mutagenesis of an industrial strain of Streptococcus thermophilus was achieved through an adapted version of a two-plasmid system. The mutagenesis strategy is based on random integration of derivatives of the non-replicative (Rep(-)) plasmid pORI19 by means of homologous recombination following a temperature shift that eliminates replication of the temperature-sensitive (Rep(ts)) helper plasmid pVE6007. In this way mutants were generated which were affected in bacteriophage sensitivity or sucrose metabolism. Homologues were identified of a protein related to folate metabolism from a bacteriophage-resistant mutant and of two subunits of an oligopeptide transport system from a mutant deficient in sucrose utilisation. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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