Peer-Reviewed Journal Details
Mandatory Fields
Levis, J,Kenny-Walsh, E,O'Sullivan, K,Horgan, M,Whelton, M,Shanahan, F,Fanning, L;
2001
January
Rna-A Publication of The Rna Society
Strategy for the maximization of clinically relevant information from hepatitis C virus, RT-PCR quantification
Validated
()
Optional Fields
hepatitis C virus viral load quantification linear range PCR cost benefit DNA SIGNAL AMPLIFICATION HCV-RNA INTERFERON TREATMENT PROBE ASSAY RIBAVIRIN COMBINATION INDIVIDUALS INFECTION FEATURES DISEASE
20
163
171
Background: The increasing clinical application of viral load assays for monitoring viral infections has been an incentive for the development of standardized tests for the hepatitis C virus. Objective : To develop a simple model for the prediction of baseline viral load in individuals infected with the hepatitis C virus. Methodology: Viral load quantification of each patient's first sample was assessed by RT-PCR-ELISA using the Roche MONITOR assay in triplicate. Genotype of the infecting virus was identified by reverse line probe hybridization. using amplicons resulting from the qualitative HCV Roche AMPLICOR assay. Results: Retrospective evaluation of first quantitative values suggested that 82.4% (n = 168/204) of individuals had a viral load between 4.3 and 6.7 log(10) viral copies per mi. A few patients (3.4%; n = 7/204) have a serum viremia less than the lower limit of the linear range of the RT-PCR assay. Subsequent, prospective evaluation of hepatitis C viral load of all new patients: using a model based on the dynamic range of viral load in the retrospective group correctly predicted the dynamic range in 75.9% (n = 33/54). Conclusion: The dynamic range of hepatitis C viremia extends beyond the linear range of the Roche MONITOR assay. Accurate determination of serum viremia is substantially improved by dilution of specimens prior to quantification. (C) 2001 Elsevier Science B.V. All rights reserved.
http://dx.doi.org/10.1016/S1386-6532(00)00177-3
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