Peer-Reviewed Journal Details
Mandatory Fields
Finucane, DM,Waterhouse, NJ,Amarante-Mendes, GP,Cotter, TG,Green, DR;
1999
August
Cell
Collapse of the inner mitochondrial transmembrane potential is not required for apoptosis of HL60 cells
Validated
()
Optional Fields
apoptosis permeability transition mitochondria caspases cytochrome c CULTURED RAT HEPATOCYTES BACULOVIRUS P35 PROTEIN CYTOCHROME-C RELEASE ICE FAMILY PROTEASES NERVE GROWTH-FACTOR PERMEABILITY TRANSITION DNA FRAGMENTATION NEURONAL DEATH THYMOCYTE APOPTOSIS LIVER-MITOCHONDRIA
251
166
174
Apoptotic cell death involves a series of morphological and biochemical changes orchestrated by activated proteases belonging to the caspase family. Recent studies have suggested that the activation of this process of execution is dependent upon events associated with the loss of mitochondrial inner transmembrane potential (Delta psi(m)), as a consequence of the formation of the permeability transition (PT) pore. This has led to the proposal that mitochondrial depolarization represents a central irreversible checkpoint in the apoptotic program. Here, we present evidence that HL-60 cells undergo apoptosis in response to the cytotoxic insults of actinomycin-D, etoposide, and staurosporine without showing significant changes in Delta psi(m) Instead, the loss of Delta psi(m) could be detected only later in the cell death pathway. In addition, the uncoupling agent CCCP produced an early mitochondrial depolarization in HL-60s but these cells showed few signs of apoptosis up to 8 h after the insult. Furthermore, examination of these cells in response to staurosporine revealed the release of mitochondrial cytochrome c into the cytosol over time, corresponding to caspase activation irrespective of mitochondrial depolarization. In summary, our data suggest that the collapse of Delta psi(m) as a consequence of PT is not a universal early marker for apoptosis and, moreover, it is not part of the central apoptotic machinery. (C) 1999 Academic Press.
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