Pseudomonas fluorescens F113, which produces the antimicrobial compound 2,4-diacetylphloroglucinol, is a prospective biocontrol agent. Soil enzyme activities were used to investigate the ecological impact of strain F113 in the rhizosphere of field-grown sugar beet. There were distinct trends in rhizosphere enzyme activities in relation to soil chemistry [determined by electro-ultrafiltration (EUF)]. The activities of enzymes from the P cycle (acid phosphatase, alkaline phosphatase and phosphodiesterase) and of arylsulphatase were negatively correlated with the amount of readily available P, whereas urease activity was positively correlated with the latter. Significant correlations between nutrient levels determined by EUF and enzyme activities in the rhizosphere were obtained, highlighting the usefulness of enzyme assays to document variations in soil nutrient cycling. Contrary to previous microcosm studies, which did not investigate plants grown to maturity, the biocontrol inoculant had no effect on enzyme activities or on soil chemistry in the rhizosphere. The results showed the importance of using homogenous soil microcosm systems, also employed in previous work, for risk assessment studies, whereby the effects of inherent soil variability were minimised, and effects of the pseudomonad on soil enzymology could be detected.