Peer-Reviewed Journal Details
Mandatory Fields
MCGARRY, A,LAW, J,COFFEY, A,DALY, C,FOX, PF,FITZGERALD, GF;
1994
December
Applied and Environmental Microbiology
EFFECT OF GENETICALLY MODIFYING THE LACTOCOCCAL PROTEOLYTIC SYSTEM ON RIPENING AND FLAVOR DEVELOPMENT IN CHEDDAR CHEESE
Validated
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Optional Fields
LACTIS SUBSP-LACTIS STARTER BACTERIA GOUDA CHEESE RENNET MILK ENZYMES GENE STREPTOCOCCI PROTEINASES MANUFACTURE
60
4226
4233
Three batches of six Cheddar cheeses mere manufactured by using the following lactococcal strains: (i) UC317 as a control; (ii) JL3601, a proteinase-negative derivative of UC317 transformed with high-copy-number plasmid pCI3601 containing the cloned proteinase gene complex from UC317; (iii) AM312, a proteinase-negative derivative of UC317 transformed with plasmid pMG36enpr containing the neutral proteinase gene from Bacillus subtilis; (iv) AC322, JL3601 transformed with pMG36enpr; (v) AC311, UC317 transformed with plasmid pNZ1120, which contains the aminopeptidase N (pepN) gene from Lactococcus lactis subsp. lactis MG1363; and (vi) AC321, JL3601 transformed with pNZ1120. Organoleptic and chemical analyses indicated that (i) the control cheeses, which were made with UC317, were of the highest quality; (ii) cheeses made with strains harboring pCI3601 in addition to either pMG36enpr (AC322) or pNZ1120 (AC321) did not ripen in a significantly different manner than cheeses made with AM312 (containing only pMG36enpr) or AC311 (containing only pNZ112O), respectively; (iii) cheeses made with strains that overproduce pepN did not have improved body, texture, and flavor characteristics; and (iv) cheeses made with strains harboring the neutral proteinase from B. subtilis (AM312 and AC322) underwent greatly accelerated proteolysis.
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