Peer-Reviewed Journal Details
Mandatory Fields
MCSWEENEY, PLH,OLSON, NF,FOX, PF,HEALY, A,HOJRUP, P;
1993
Food Biotechnology
PROTEOLYTIC SPECIFICITY OF PLASMIN ON BOVINE ALPHA-S1-CASEIN
Validated
()
Optional Fields
BETA-CASEIN MILK CHEESE ALPHA-S2-CASEIN PROTEINS
7
143
158
The proteolytic specificity of plasmin (fibrinolysin, E. C. 3.4.21.7, from bovine plasma) on bovine alphas1-casein was determined in solution in 50 mM ammonium bicarbonate buffer, pH 8.4, at 37-degrees-C.Peptides, isolated by reverse-phase high performance liquid chromatography on a C18 column or by electroblotting from urea-polyacrylamide gel electrophoretograms, were identified from their amino acid sequence and mass.The principal plasmin cleavage sites were found at Arg22-Phe23, Arg90-Tyr91, Lys102-Lys103, Lys103-Tyr104, Lys105-Val106, Lys124-Glu125 and Arg151-Gln152. The initial cleavage sites and the order of production of small (pH 4.6-soluble) peptides suggest that alphas1-casein was cleaved initially towards the centre of the molecule.
Grant Details