Peer-Reviewed Journal Details
Mandatory Fields
O'Sullivan, D.J.,Dowling, D.N.,deLorenzo, V.,O'Gara, F. ;
1994
April
Fems Microbiology Letters
Escherichia coli ferric uptake regulator (Fur) can mediate regulation of a pseudomonad iron-regulated promoter
Validated
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Optional Fields
PSEUDOMONAS IRON-REGULATED PROMOTER FUR FOOT-PRINTING FLUORESCENT PSEUDOMONAD GENE TRANSCRIPTION REPRESSOR BINDING PROTEIN
117
327
332
Introduction of a Pseudomonas iron-regulated promoter lacZ fusion (SP1) and a Pseudomonas transcriptional factor into Escherichia coli allowed expression of the promoter in this heterologous host. Evaluation of this promoter in wild-type and fur mutants of E. coli, by measuring beta-galactosidase activity, indicated that E. coli Fur can regulate the Pseudomonas promoter in response to iron starvation. Gel retardation assays suggested that purified Fur protein could interact with the SP1 promoter upstream of the transcriptional start. DNase I footprinting analysis established that Fur protected a primary 58-bp region (-50 to -106 bp). These protein/DNA interactions correlate with the observed in vivo regulation of the SP1 promoter in E. coli and indicate that Fur can functionally regulate a Pseudomonas iron-regulated promoter.
Grant Details