Peer-Reviewed Journal Details
Mandatory Fields
FOLEY, S,DALY, C,FITZGERALD, GF;
1993
June
Journal of General Microbiology
INCOMPATIBILITY PROPERTIES OF THE NARROW-HOST-RANGE LACTOCOCCAL PLASMID PCI305
Validated
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Optional Fields
STREPTOCOCCUS-LACTIS BACILLUS-SUBTILIS SEGREGATIONAL INSTABILITY STAPHYLOCOCCAL PLASMIDS BACTERIAL PLASMIDS MINIMAL REPLICON SHUTTLE VECTOR REPLICATION DNA CLONING
139
1271
1276
The cryptic plasmid pCI305 from Laetococcus lactis subsp. lactis UC317 was screened for incompatibility with a range of cloning vectors and was found to be incompatible with the theta-replicating plasmids pAM401 and pIL253. In contrast, lactococcal vectors containing the replication functions of the rolling circle replicating plasmids pSH71 and pWV01 were compatible with pCI305. A number of native lactococcal plasmids were also screened for both incompatibility and DNA sequence homology to the pCI305 replicon. A plasmid with high sequence identity was identified which was compatible with pCI305.
Grant Details