1. The effect of feeding alpha-tocopherol (5 to 180-mu-g/g diet) for 24 days on the concentrations of alpha-tocopherol in various chicken tissues was investigated.2. Tissue alpha-tocopherol concentrations responded to dietary intake in the order: heart congruent-to lung > liver > thigh muscle > brain, and in all cases the relationship between the concentrations of dietary and tissue alpha-tocopherol was highly significant (0.997 less-than-or-equal-to r less-than-or-equal-to 1).3. Plasma alpha-tocopherol concentration appears to be a good index (r greater-than-or-equal-to 0.910, P < 0.001) of alpha-tocopherol status of lung, liver and heart.