Peer-Reviewed Journal Details
Mandatory Fields
Choudhary Alka; Naughton Lynn M.; Dobson Alan D.W.; Rai Dilip K.
2018
July
Rapid Communications In Mass Spectrometry
HPLC-ESI-MS/MS characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge-derived bacterium isolated from Irish waters
Validated
Optional Fields
Pseudovibrio sp. Tropodithietic acid Cholic acid Sphinganine HPLC-MS/MS
Rationale: In recent years, metabolites produced by Pseudovibrio species have gained scientific attention due to their potent antimicrobial activity. Recently we also have assessed antibacterial activities of Pseudovibrio sp. W64 isolates against Staphylococcus aureus, where only the dominant tropodithietic acid (TDA) was identified. However characterisation of other metabolites is necessary as these metabolites may also serve as potent antimicrobial agents. Methods: LC-MS/MS, aided by accurate mass measurements, was employed to screen and characterise a range of metabolites produced by Pseudovibrio sp. W64 via assessment of ethyl acetate fractions generated from bacterial cultures. Results: Thirteen metabolites unique to the bacterial culture were detected and their chemical structures were assigned by tandem mass spectrometry and accurate mass measurement. Among the thirteen metabolites, a methyl ester of TDA, a number of cholic acid derivatives, and amino diols and triols were characterised. Conclusions: Pseudovibrio sp. W64 produces methylated TDA in addition to TDA, and metabolises lipids and amino acids in the cell-culture medium. To the best of our knowledge, this is the first report of methylated TDA, cholic acid and its various analogs, and sphinganine being detected in this Pseudovibrio strain. The data generated may help to better understand the biochemical processes and metabolism of bacterial strains towards discovery of antimicrobial agents from marine sources.
0951-4198
https://onlinelibrary.wiley.com/doi/abs/10.1002/rcm.8226
10.1002/rcm.8226
Grant Details