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Mandatory Fields
Reviews
Papkovsky, DB;Dmitriev, RI
2018
August
Imaging of oxygen and hypoxia in cell and tissue samples
Validated
1
Optional Fields
GREEN FLUORESCENT PROTEIN LUMINESCENCE LIFETIME SPECTROSCOPY TIME-RESOLVED FLUOROMETRY IN-VIVO INTRACELLULAR OXYGEN PHOSPHORESCENT PROBES MAMMALIAN-CELLS TUMOR HYPOXIA PHOTODYNAMIC THERAPY LIVING CELLS
Molecular oxygen (O-2) is a key player in cell mitochondrial function, redox balance and oxidative stress, normal tissue function and many common disease states. Various chemical, physical and biological methods have been proposed for measurement, real-time monitoring and imaging of O-2 concentration, state of decreased O-2 (hypoxia) and related parameters in cells and tissue. Here, we review the established and emerging optical microscopy techniques allowing to visualize O-2 levels in cells and tissue samples, mostly under in vitro and ex vivo, but also under in vivo settings. Particular examples include fluorescent hypoxia stains, fluorescent protein reporter systems, phosphorescent probes and nanosensors of different types. These techniques allow high-resolution mapping of O-2 gradients in live or post-mortem tissue, in 2D or 3D, qualitatively or quantitatively. They enable control and monitoring of oxygenation conditions and their correlation with other biomarkers of cell and tissue function. Comparison of these techniques and corresponding imaging setups, their analytical capabilities and typical applications are given.
BASEL
SPRINGER BASEL AG
1420-682X
2963
2980
10.1007/s00018-018-2840-x
Grant Details