Peer-Reviewed Journal Details
Mandatory Fields
Baumann, L;Rajkumar, AS;Morrissey, JP;Boles, E;Oreb, M
2018
November
ACS Synthetic Biology
A Yeast-Based Biosensor for Screening of Short- and Medium-Chain Fatty Acid Production
Validated
Optional Fields
TRANSCRIPTION FACTOR WAR1 SACCHAROMYCES-CEREVISIAE ABC TRANSPORTER STRESS-RESPONSE RESISTANCE
7
2640
2646
Short-and medium-chain fatty acids (SMCFA) are important platform chemicals currently produced from non sustainable resources. The engineering of microbial cells to produce SMCFA, however, lacks high-throughput methods to screen for best performing cells. Here, we present the development of a whole-cell biosensor for easy and rapid detection of SMCFA. The biosensor is based on a multicopy yeast plasmid containing the SMCFA-responsive PDR12 promoter coupled to GFP as the reporter gene. The sensor detected hexanoic, heptanoic and octanoic acid over a linear range up to 2, 1.5, and 0.75 mM, respectively, but did not show a linear response to decanoic and dodecanoic acid. We validated the functionality of the biosensor with culture supernatants of a previously engineered Saccharomyces cerevisiae octanoic acid producer strain and derivatives thereof. The biosensor signal correlated strongly with the octanoic acid concentrations as determined by gas chromatography. Thus, this biosensor enables the high-throughput screening of SMCFA producers and has the potential to drastically speed up the engineering of diverse SMCFA producing cell factories.
WASHINGTON
2161-5063
10.1021/acssynbio.8b00309
Grant Details