Peer-Reviewed Journal Details
Mandatory Fields
Guerin, E;Shkoporov, A;Stockdale, SR;Clooney, AG;Ryan, FJ;Sutton, TDS;Draper, LA;Gonzalez-Tortuero, E;Ross, RP;Hill, C
2018
November
Cell Host &Amp; Microbe
Biology and Taxonomy of crAss-like Bacteriophages, the Most Abundant Virus in the Human Gut
Validated
WOS: 112 ()
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24
653
CrAssphages represent the most abundant virus in the human gut microbiota, but the lack of available genome sequences for comparison has kept them enigmatic. Recently, sequence-based classification of distantly related crAss-like phages from multiple environments was reported, leading to a proposed familial-level taxonomic group. Here, we assembled the metagenomic sequencing reads from 702 human fecal virome/phageome samples and analyzed 99 complete circular crAss-like phage genomes and 150 contigs >= 70 kb. In silico comparative genomics and taxonomic analysis enabled a classification scheme of crAss-like phages from human fecal microbiomes into four candidate subfamilies composed of ten candidate genera. Laboratory analysis was performed on fecal samples from an individual harboring seven distinct crAss-like phages. We achieved crAss-like phage propagation in ex vivo human fecal fermentations and visualized short-tailed podoviruses by electron microscopy. Mass spectrometry of a crAss-like phage capsid protein could be linked to metagenomic sequencing data, confirming crAss-like phage structural annotations.
CAMBRIDGE
1931-3128
10.1016/j.chom.2018.10.002
Grant Details