Peer-Reviewed Journal Details
Mandatory Fields
Grebenik, EA;Istranov, LP;Istranova, EV;Churbanov, SN;Shavkuta, BS;Dmitriev, RI;Veryasova, NN;Kotova, SL;Kurkov, AV;Shekhter, AB;Timashev, PS
2019
May
Xenotransplantation
Chemical cross-linking of xenopericardial biomeshes: A bottom-up study of structural and functional correlations
Validated
Optional Fields
BOVINE PERICARDIUM EXTRACELLULAR-MATRIX MECHANICAL-PROPERTIES PORCINE PERICARDIUM BONE REGENERATION IN-VITRO TISSUE SCAFFOLD MESH DECELLULARIZATION
26
Decellularized bovine pericardium (DBP)-based biomeshes are the gold standard in reconstructive surgery. In order to prolong their stability after the transplantation, various chemical cross-linking strategies are employed. However, structural and functional properties of the biomeshes differ in dependence on the cross-linker used. Here, we performed a bottom-up study of structural and functional alterations of DBP-based biomeshes following cross-linking with hexamethylene diisocyanate (HMDC), ethylene glycol diglycidyl ether (EGDE), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and genipin. The in vitro cytotoxicity tests supported their clinical applicability. Their structural differences (eg roughness, fibre thickness, pore morphology) were evaluated using the two-photon confocal laser scanning, atomic force, scanning electron and polarized light microscopies. HMDC and EDC samples appeared to be the roughest. Complex mechanical trials indicated the tendency to reduced Young's Modulus and mechanical anisotropy values of DBP upon cross-linking. The lowest mechanical anisotropy was found in EDC and genipin sample groups. In vitro collagenase susceptibility was the highest for EDC samples and the lowest for EGDE samples. The comparative analysis of the results allowed us to recognize the strengths and weaknesses of each cross-linker in relation to a particular clinical application.
HOBOKEN
0908-665X
10.1111/xen.12506
Grant Details