Peer-Reviewed Journal Details
Mandatory Fields
Considine K.;Sleator R.;Kelly A.;Fitzgerald G.;Hill C.
Bioengineered Bugs
Identification and characterization of an essential gene in Listeria monocytogenes using an inducible gene expression system
Scopus: 8 ()
Optional Fields
Essential gene EzrA High pressure Listeria monocytogenes
The Listeria monocytogenes gene lmo1594 is a homolog of the Bacillus subtilis cell division gene ezrA. EzrA is a negative regulator of FtsZ ring formation, which is required for efficient cell division as it regulates the frequency and position of Z-rings in the cell and prevents aberrant polar cell division. Previously identified as a putative high pressure (HP) resistance mechanism; conferring enhanced barotolerance when heterologously expressed against an Escherichia coli background; the aim of the current study was to investigate whether lmo1594 plays a role in listerial barotolerance. When the creation of a deletion mutant proved unsuccessful, the role of lmo1594 was addressed by creating a conditional knockout mutant which demonstrated that the gene is in fact essential for cell survival and growth in L. monocytogenes. In order to investigate the effect of lmo1594 on barotolerance, the gene was overexpressed. The overexpression of lmo1594 increased survival levels in L. monocytogenes treated at 300 MPa, but survival levels similar to those of the wildtype strain were observed when treated at a higher pressure (=400 MPa). In conclusion, this study reveals for the first time that lmo1594 is absolutely essential for listerial cell growth and survival, and also plays an important role in listerial barotolerance. 2011 Landes Bioscience.
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