Peer-Reviewed Journal Details
Mandatory Fields
McNamara, AE;Collins, C;Harsha, PSCS;Gonzalez-Pena, D;Gibbons, H;McNulty, BA;Nugent, AP;Walton, J;Flynn, A;Brennan, L
2020
April
Molecular Nutrition & Food Research
Metabolomic-Based Approach to Identify Biomarkers of Apple Intake
Validated
Optional Fields
URINARY BIOMARKERS REPORT INSTRUMENTS VEGETABLES FRUITS IDENTIFICATION CONSUMPTION FLAVONOIDS STRATEGY ACIDS RISK
Scope There is an increased interest in developing biomarkers of food intake to address some of the limitations associated with self-reported data. The objective is to identify biomarkers of apple intake, examine dose-response relationships, and agreement with self-reported data.Methods and results Metabolomic data from three studies are examined: an acute intervention, a short-term intervention, and a free-living cohort study. Fasting and postprandial urine samples are collected for analysis by H-1-NMR and liquid chromatography-mass spectrometry (LC-MS). Calibration curves are developed to determine apple intake and classify individuals into categories of intake. Multivariate analysis of data reveals that levels of multiple metabolites increase significantly post-apple consumption, compared to the control food-broccoli. In the dose-response study, urinary xylose, epicatechin sulfate, and 2,6-dimethyl-2-(2-hydroxyethyl)-3,4-dihydro-2H-1-benzopyran increase as apple intake increases. Urinary xylose concentrations in a free-living cohort perform poorly at an individual level but are capable of ranking individuals in categories of intake.Conclusion Urinary xylose exhibits a dose-response relationship with apple intake and performs well as a ranking biomarker in the population study. Other potential biomarkers are identified and future work will combine these with xylose in a biomarker panel which may allow for a more objective determination of individual intake.
HOBOKEN
1613-4125
10.1002/mnfr.201901158
Grant Details