© 2019 Human Kinetics, Inc. The aim of this study was to quantify levels of physical activity (PA) and fundamental movement skills (FMS) of children aged 3 to 5 years in Irish preschool services during care hours, and investigate the relationship between these two variables. Data were collected from 141 children (50.3% boys, age M = 3.9 ± 0.5 years) across 9 preschool services. Measurements included PA via accelerometry, and proficiency in four FMS (run, vertical jump, throw and catch). The recommended guideline of 15 minutes of PA per hour (min PA/hour) was met by 35% of children (M = 13.6 min PA/hour). Significant differences in mean PA per hour were found by gender, with boys (14.2 min PA/hour) more active than girls (13.0 min PA/ hour), and age, with younger children (14.2 min PA/hour) more active than older (12.6 minutes PA/hour). Percentage of children proficient in the run was high (88.4%), but low across the other skills (4.9%-18.5%). Significant differences were identified by gender for vertical jump with girls scoring higher than boys. No significant relationship was found between FMS and total PA. Low levels of PA and FMS proficiency highlight need for intervention in early years settings to ensure children develop skills to participate in PA.