Peer-Reviewed Journal Details
Mandatory Fields
Queiroz A.L.P.;Kerins B.M.;Yadav J.;Farag F.;Faisal W.;Crowley M.E.;Lawrence S.E.;Moynihan H.A.;Healy A.M.;Vucen S.;Crean A.M.
2021
September
Cellulose
Investigating microcrystalline cellulose crystallinity using Raman spectroscopy
Validated
WOS: 1 ()
Optional Fields
Crystallinity Microcrystalline cellulose Partial least square regression R Shiny Raman spectroscopy
28
14
8971
8985
Microcrystalline cellulose (MCC) is a semi-crystalline material with inherent variable crystallinity due to raw material source and variable manufacturing conditions. MCC crystallinity variability can result in downstream process variability. The aim of this study was to develop models to determine MCC crystallinity index (%CI) from Raman spectra of 30commercial batches using Raman probes with spot sizes of 100m (MR probe) and 6mm (PhAT probe). A principal component analysis model separated Raman spectra of the same samples captured using the different probes. The %CI was determined using a previously reported univariate model based on the ratio of the peaks at 380 and 1096cm-1. The univariate model was adjusted for each probe. The %CI was also predicted from spectral data from each probe using partial least squares regression models (where Raman spectra and univariate %CI were the dependent and independent variables, respectively). Both models showed adequate predictive power. For these models a general reference amorphous spectrum was proposed for each instrument. The development of the PLS model substantially reduced the analysis time as it eliminates the need for spectral deconvolution. A web application containing all the models was developed. Graphic abstract: [Figure not available: see fulltext.]
0969-0239
10.1007/s10570-021-04093-1
Grant Details