Peer-Reviewed Journal Details
Mandatory Fields
Hudson, AM;Loughran, G;Szabo, NL;Wills, NM;Atkins, JF;Cooley, L
2021
February
Proceedings of The National Academy of Sciences of The United States of America
Tissue-specific dynamic codon redefinition in Drosophila
Validated
WOS: 5 ()
Optional Fields
TRANSLATIONAL TERMINATION EFFICIENCY MESSENGER-RNA DECAY STOP CODON READ-THROUGH GENE-EXPRESSION PROTEIN READTHROUGH NUCLEOTIDES DOWNSTREAM SIGNAL
118
Translational stop codon readthrough occurs in organisms ranging from viruses to mammals and is especially prevalent in decoding Drosophila and viral mRNAs. Recoding of UGA, UAG, or UAA to specify an amino acid allows a proportion of the protein encoded by a single gene to be C-terminally extended. The extended product from Drosophila kelch mRNA is 160 kDa, whereas unextended Kelch protein, a subunit of a Cullin3-RING ubiquitin ligase, is 76 kDa. Previously we reported tissue-specific regulation of readthrough of the first kelch stop codon. Here, we characterize major efficiency differences in a variety of cell types. Immunoblotting revealed low levels of readthrough in malpighian tubules, ovary, and testis but abundant readthrough product in lysates of larval and adult central nervous system (CNS) tissue. Reporters of readthrough demonstrated greater than 30% readthrough in adult brains, and imaging in larval and adult brains showed that readthrough occurred in neurons but not glia. The extent of readthrough stimulatory sequences flanking the readthrough stop codon was assessed in transgenic Drosophila and in human tissue culture cells where inefficient readthrough occurs. A 99-nucleotide sequence with potential to form an mRNA stem-loop 3' of the readthrough stop codon stimulated readthrough efficiency. However, even with just six nucleotides of kelch mRNA sequence 3' of the stop codon, readthrough efficiency only dropped to 6% in adult neurons. Finally, we show that high-efficiency readthrough in the Drosophila CNS is common; for many neuronal proteins, C-terminal extended forms of individual proteins are likely relatively abundant.
WASHINGTON
0027-8424
10.1073/pnas.2012793118
Grant Details