Peer-Reviewed Journal Details
Mandatory Fields
Daniloskia, Davor,McCarthy, Noel A.,OĺCallaghan, Tom F.,Vasiljevic, Todor
2022
International Dairy Journal
Authentication of ▀-casein milk phenotypes using FTIR spectroscopy
Validated
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Optional Fields
105350
The ability to accurately and cost-effectively distinguish between different genetic protein variants in milk is of pivotal significance to the dairy industry as the natural proteoforms of caseins affect the composition and functionality of dairy products. Recently, ▀-casein proteoforms have received increased consumer attention in regards to A1 and A2 milk families. Fourier transform infrared (FTIR) spectroscopy coupled with multivariate analysis was used to discriminate milk samples from 114 Holstein-Friesian cows possessing different ▀-casein genetic variants. Principal component and partial least squares-discriminant analyses were used to characterise and distinguish A1 and A2 ▀-casein genetic families based on spectral data. Milk containing A2 ▀-casein had higher proportions of random coil and ▀-sheet conformations than milk containing A1 ▀-casein and the lowest amount of a-helical and ▀-turn structures, due to additional proline. This may provide a feature to distinguish between these phenotypes using FTIR spectroscopy in association with chemometric analyses.
0958-6946
https://www.sciencedirect.com/science/article/pii/S0958694622000346
https://doi.org/10.1016/j.idairyj.2022.105350
Grant Details