Conference Publication Details
Mandatory Fields
Downey, J. D. & Crean, A. & Ryan, K. B.,
13th APS PharmSci International Conference
Investigating Buffer Effects on Lysozyme Adsorption to Borosilicate Glass
2022
September
Published
1
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Optional Fields
Protein Adsorption, Buffer, Stability, Borosilicate
S1
S2
Belfast
Protein adsorption refers to the accumulation and adherence of a protein to the surface of a solid, but without surface, penetration occurring. Proteins can adsorb to a variety of interfaces that are used in the manufacture, formulation, and storage of protein medicines. This can have unintended consequences such as a loss of expensive protein products and aggregate formation. This study investigates the role of buffer composition, pH, and protein concentration on the adsorption of lysozyme to borosilicate glass. Using reverse-phase HPLC and differential scanning fluorimetry, we quantified the mass of adsorbed lysozyme and assessed the stability of lysozyme in each buffer system. The highest amount of adsorbed lysozyme occurred in the sodium phosphate and histidine-HCl buffers at pH 7.4 and stability analysis showed that lysozyme had the lowest melt temperature in these buffers.
European Regional Development Fund and EPSRC-CDT and the Engineering and Physical Sciences Research Council UK
doi: https://doi.org/10.5920/bjpharm.1157
Grant Details
Science Foundation Ireland
European Regional Development Fund and EPSRC-CDT and the Engineering and Physical Sciences Research Council UK