Peer-Reviewed Journal Details
Mandatory Fields
Anoopkumar-Dukie S, McMahon A, Allshire A, Conere TJ;
2005
April
The British Journal of Radiology
Further evidence for biological effects resulting from ionizing radiation doses in the diagnostic X-ray range.
Validated
WOS: 7 ()
Optional Fields
78
928
335
337
In this study we assessed cell response to ionizing radiation doses in the diagnostic X-ray range, using metabolic performance as the biological end point. HeLa cells were irradiated between 3.5 mGy (standard deviation (SD=2.5%) and 55.1 mGy (SD=0.9%) with 110 kV X-rays, and after 7 days metabolic capacity of cultures was measured spectrophotometrically with resazurin. Cells receiving 7.5 mGy or greater showed impaired proliferation. At 7.5 mGy this effect was prevented by nitric oxide synthase inhibitor N G-monomethyl-L-arginine but not by ascorbic acid or p38 MAPK inhibitor SB203580. We conclude that HeLa cell proliferation is adversely affected by doses within the range of some radiological examinations, through a mechanism involving nitric oxide.
10.1259/bjr/24426084
Grant Details